CAS:7385-67-3 | Nile Red

Name:Nile Red

Synonyms:9-(Diethylamino)-5H-benzo[a]phenoxazin-5-one

CAS:7385-67-3

Purity:98%

Molecular Formula:C₂₀H₁₈N₂O₂

Molecular Weight:318.38 

Melting Point:203-205° C (lit.)

Boiling Point:484.70° C at 760 mmHg (Predicted)

Density:1.2 g/cm³ (Predicted)

Appearance:Yellow to Brown to Dark green powder to crystal

Solubility:Soluble in water (Partly miscible), and methanol (1 mg/ml)

Acidity coefficient:4.08 (Predicted)

refractive index:1.64 (Predicted)

Absorbance(E1%1cm):min. 1,300(MeOH, 550.0 to 554.0 nm)

Fluorescent:λex 552 nm, λem 636 nm

Storage condition:Store at room temperature

Application:Nile Red(CAS:7385-67-3) is an uncharged, hydrophobic, photostable molecule that strongly fluoresces bright red in hydrophobic environments. Nile Red is a lipophilic stain commonly used for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytofluorometry. Nile Red has been shown to detect the exposure or formation of new hydrophobic surfaces induced by ligand binding to calmodulin, oligomerization of melittin, or unfolding of ovalbumin during early thermal denaturation. Nile Red has been employed to stain cell structures, as well as to visualize and localize colloidal drug carriers. Nile Red′s fluorescence is unaffected between pH 4.5 and 8.5 and may be used in dilute concentrations to investigate hydrophobic protein sites.

Reference: 

1. Greenspan, P., et al. 1985. J. Cell Biol. 100: 965-973. PMID: 3972906

2. Sackett, D.L. and Wolff, J. 1987. Anal. Biochem. 167: 228-234. PMID: 3442318

3. Lamprecht, A. et al. 2003. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 787: 415-419. PMID: 12650763

4. Makino, A., et al. 2006. Biochemistry. 45: 4530-4541. PMID: 16584188

5. Werner, T., et al. 2006. Cytometry A. 69: 200-202. PMID: 16496419

6. Varghese, R., et al. 2010. Chemistry. 16: 9040-9046. PMID: 20645354

Packaging & Delivery

Package:50g/100g/250g/500g/1kg/bulk package

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