CAS:104821-25-2 | Dihydroethidium

Name:Dihydroethidium

Synonyms:Dihydroethidium also known as Hydroethidine; PD-MY 003; 5-Ethyl-5,6-dihydro-6-phenyl-3,8-phenanthridinediamine; (±)-5-Ethyl-5,6-dihydro-6-phenyl-3,8-phenanthridinediamine

CAS:104821-25-2

Purity:98%

Molecular Formula:C₂₁H₂₁N₃

Molecular Weight:315.41

Melting Point:214.14° C (Predicted)

Boiling Point:580.4° C at 760 mmHg (Predicted)

Density:1.20 g/cm³ (Predicted)

Appearance:Pink to dark brown powder

Solubility:Soluble in DMSO, DMF, acetonitrile, methanol, and chloroform (20 mg/ml)

Acidity coefficient:5.30±0.40(Predicted)

refractive index:1.68 (Predicted)

Fluorescent:λex 358 nm, λem 461 nm; λex 392 nm, λem 410 nm in methanol

Storage condition:Store at -20° C

Application:Dihydroethidium(CAS:104821-25-2) is also known as hydroethidine. Dihydroethidium is reduced form of the commonly used DNA dye ethidium bromide. Dihydroethidium is a dye that can permeate viable cells and accumulate in the nucleus after it is dehydrogenated to ethidium bromide and is used to measure oxygen reduction in phagocytes during respiratory burst. In the presence of an oxidant Dihydroethidium will fluoresce and has been used as a dye in flow cytometry. Studies have used Dihydroethidium to study alveolar macrophages, blood leukocytes, and epithelial cells, among others, and has be used to measure intracellular H2O2 and O2-, and reactive oxygen.

Reference: 

1. Bucana, C.D., et al. 1990. Exp. Cell Res. 190: 69-75. PMID: 2387329

2. Rothe, G. and Valet, G. 1990. J. Leukoc. Biol. 47: 440-448. PMID: 2159514

3. Perticarari, S., et al. 1991. Cytometry. 12: 687-693. PMID: 1782835

4. Carter, W.O., et al. 1994. J. Leukoc. Biol. 55: 253-258. PMID: 8301222

5. Mancini, M., et al. 1997. J. Cell Biol. 138: 449-469. PMID: 9230085

6. Haugen, T.S., et al. 1999. Eur. Respir. J. 14: 1100-1105. PMID: 10596697 

Packaging & Delivery

Package:5mg/10mg/25mg/50mg/bulk package

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