CAS:148504-34-1 | Calcein-AM

Name:Calcein-AM

Synonyms:N,N′-[[3′,6′-Bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9′-[9H]xanthene]-2′,7′-diyl]bis(methylene)]bis[N-[2-[(acetyloxy)methoxy]-2-oxoethyl]glycine 1,1′-Bis[(acetyloxy)methyl] Ester; Calcein Acetoxymethyl Ester; NSC 689290

CAS:148504-34-1

Purity:95%

Molecular Formula:C₄₆H₄₆N₂O₂₃

Molecular Weight:994.86

Melting Point:N/A

Boiling Point:982.73° C at 760 mmHg (Predicted)

Flash Point:85 °C

Density:1.49 g/cm³ (Predicted)

Appearance:Crystalline Solid

Solubility:Soluble in DMSO (10 mg/ml)

Acidity coefficient:2.66±0.50(Predicted)

refractive index:1.61 (Predicted)

Fluorescent:λex 496 nm, λem 516 nm in 0.1 M Tris pH 8.0

Storage condition:Desiccate at -20° C

Application:Calcein-AM(CAS:148504-34-1) is a hydrophobic non-fluorescent probe that can permeate the plasma membrane and can be hydrolyzed to calcein (sc-202090). Calcein-AM is an extremely fluorescent and negatively charged molecule. The Calcein-AM probe can be used to stain living cells and has been observed to fluoresce bright green in cytoplasmic Schwann cells. Calcein-AM has been reported to be a neutral substrate for Mdr (P-glycoprotein (Pgp) and multidrug resistance protein (MRP)) and has been used in flow cytometry studies to analyze the function of P-gp and MRP. Studies suggest that Calcein-AM can be used to detect MDR mediated resistance and to be transported by PGP from the plasma membrane. Mitochondrial permeability research has utilized Calcein-AM as a cytosolic fluorophore. Calcein-AM has been used as a cytosolic fluorophore in mitochondrial permeability studies to image pore transition.

Reference: 

1. Feller, N., et al. 1995. Br. J. Cancer. 72: 543-549. PMID: 7669559

2. Feller, N., et al. 1995. FEBS Lett. 368: 385-388. PMID: 7628644

3. Essodaigui, M., et al. 1998. Biochemistry. 37: 2243-2250. PMID: 9485370

4. Lemasters, J.J., et al. 1998. Biochim. Biophys. Acta. 1366: 177-196. PMID: 9714796

5. Dhar, S., et al. 1998. Eur. J. Pharmacol. 346: 315-322. PMID: 9652375 

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