Application Of N-Tris (Hydroxymethyl) Methylglycine
Nov 09, 2021
N-Tris(hydroxymethyl)methylglycine, Tricine for short, is a zwitterionic buffer used in biochemistry and molecular biology. The pH buffer range of Tricine is 7.4-8.8. It is mainly used to replace glycine. It is used in combination with SDS in small molecule protein electrophoresis. In biochemical research, it is often used in biochemical diagnostic kits, DNA/RNA extraction kits and PCR diagnostic kits. inside.
In biochemical diagnostic kits, DNA/RNA extraction kits and PCR diagnostic kits, N-tris(hydroxymethyl)methylglycine can inhibit the activity of nucleases and maintain cell osmotic pressure. It can still maintain its integrity and high quality afterwards, and can be used for long fragment amplification. It has a major contribution. In addition, N-tris(hydroxymethyl)methylglycine also has a broad-spectrum antibacterial effect.
N-tris(hydroxymethyl)methylglycine is used as a chelating agent to chelate with the main chelating agent hydrazinobenzinamide.
In addition, Tricine can also be used as an amphoteric electrolyte to prepare polyacrylamide gels. Polyacrylamide gels are widely used in biotechnology laboratories to process biological samples to separate the biomolecules present in the samples for use Identify, and in some cases, quantitatively separate substances.
