CAS:23491-45-4 | Bisbenzimide H 33258 Fluorochrome, Trihydrochloride

Name:Bisbenzimide H 33258 Fluorochrome, Trihydrochloride

Synonyms:2'-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bibenzimidazole Trihydrochloride Hydrate;Bisbenzimide H 33258 Hydrate

CAS:23491-45-4

Purity:98%

Molecular Formula:C₂₅H₂₄N₆O•₃HCl

Molecular Weight:533.88

Melting Point:314 °C 

Boiling Point:N/A

EINECS:245-690-6 

Appearance:Dark yellow to tan may have green cast powder

Solubility:DMSO: 20 mg/ml;PBS (pH 7.2): 5 mg/ml

Emission:461 nm

Excitation:352 nm

Storage condition:2~8°C

InChi:InChI=1S/C25H24N6O.3ClH/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16;;;/h2-9,14-15,26,28H,10-13H2,1H3,(H,27,29);3*1H

InChi Key:OKQAASMPIOSOEX-UHFFFAOYSA-N

Application:Bisbenzimide H 33258 Fluorochrome, Trihydrochloride (CAS:23491-45-4) is a membrane-permeable, adenine-thymine-specific fluorescent stain, that is useful for staining DNA, chromosomes, and nuclei. This product has been shown to bind to the minor groove in DNA and can be used to detect mycoplasma contamination in tissue culture, to indicate early onset of apoptosis, and to sort living cells based on DNA content. H 33258 and the related compound H 33342 (sc-200908) have been used in conjunction with RNA aptamers in the 5′ untranslated region (UTR) to control expression of transfected gene constructs, and both have been shown to affect the rate of channel-mediated Ca2+ efflux from junctional cytoplasmic reticulum vesicles; interestingly H 33258 increases eflux while H 33342 (sc-200908) decreases it.

Reference: 

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3. Jin, R., et al. 1988. Proc. Natl. Acad. Sci. U.S.A. 85: 8939-8942. PMID: 2461559

4. Oberhammer, F., et al. 1993. EMBO J. 12: 3679-3684. PMID: 8253089

5. Papadimitriou, E., et al. 1993. J. Immunol. Methods. 162: 41-45. PMID: 7685366

6. Beeler, T.J., et al. 1993. J. Membr. Biol. 135: 109-118. PMID: 7692064

7. Battaglia, M., et al. 1994. Biotech Histochem. 69: 152-156. PMID: 7520758

8. Elstein, K.H., et al. 1994. Exp. Cell Res. 211: 322-331. PMID: 7511539

9. Tarshis, M., et al. 1994. J. Immunol. Methods. 168: 245-252. PMID: 7508483

10. Werstuck, G., et al. 1998. Science. 282: 296-298. PMID: 9765156

Packaging & Delivery

Package:100mg/250mg/500mg/bulk package

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