CAS:1239-45-8 | Ethidium Bromide

Name:Ethidium bromide

Synonyms:EtBr; Dromilac; 3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide

CAS:1239-45-8

Purity:98%

Molecular Formula:C₂₁H₂₀N₃•Br

Molecular Weight:394.3

Melting Point:260-262° C (lit.)(dec.)

Boiling Point:N/A

Density:1.3739g/cm³ (rough estimate)

Appearance:Very Dark Red to Dark Purple Solid

Stability:Stable. Incompatible with strong oxidizing agents.

Solubility:water (10 mg/mL),DMSO (Slightly), Methanol (Slightly), Water (Slightly, Sonicated)

Fluorescent:λex 480 nm, λem 620 nm in water

refractive index:1.6700 (estimate)

Storage condition:Store at room temperature

Application:Ethidium bromide(CAS:1239-45-8) is a polyaromatic fluorescent small molecule and nucleic acid intercalator widely used as a stain in DNA and RNA electrophoresis. Upon intercalation into the hydrophobic region between nucleic acid base pairs, fluorescence-quenching water molecules associated to the Ethidium bromide structure are displaced and the bound Ethidium bromide then fluoresces very strongly compared to free Ethidium bromide (~25 fold increase in dsDNA). Intercalation distorts the ribonucleic acid structure and impedes natural DNA interactions, presenting a powerful mutagen effect associated with Ethidium bromide. This imposition on DNA structure also makes Ethidium bromide an inhibitor of DNA polymerase.

Reference: 

 1. Elliott, W.H. 1963. Biochem. J. 86: 562-567. PMID: 16748992

2. Lepecq, J.B., et al. 1964. C.R. Hebd. Seances Acad. Sci. 259: 1786-1789. PMID: 14196160

3. LePecq, J.B. and Paoletti, C. 1967. J. Mol. Biol. 27: 87-106. PMID: 6033613

4. Douthart, R.J., et al. 1973. Biochemistry. 12: 214-220. PMID: 4630220

5. El Hamalawi, A.R., et al. 1975. Anal. Biochem. 67: 384-391. PMID: 1163763

6. Karsten, U. and Wollenberger, A. 1977. Anal. Biochem. 77: 464-470. PMID: 842829

7. Laine, B., et al. 1984. Biochem. Biophys. Res. Commun. 119: 1147-1153. PMID: 6370250

8. Moore, S.P. and Sutherland, B.M. 1985. Anal. Biochem. 144: 15-19. PMID: 2858989

9. Scaria, P.V. and Shafer, R.H. 1991. J. Biol. Chem. 266: 5417-5423. PMID: 2005088

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