CAS:27072-45-3 | Fluorescein 5(6)-isothiocyanate

Name:Fluorescein 5(6)-isothiocyanate

Synonyms:5(6)-Fluorescein isothiocyanate, FITC, Fluorescein-5(6)-isothiocyanate

CAS:27072-45-3

Purity:≥97%

Molecular Formula:C₂₁H₁₁NO₅S

Molecular Weight:389.38

Melting Point:>360ºC(lit.)

Boiling Point:708.6ºC at 760mmHg

Flash Point:382.4ºC

Density:1.54g/cm³

PSA:120.44

LogP:4.40

Appearance:Yellow green powder

Solubility:Soluble in DMSO (5 mg/ml), methanol, DMF, and ethanol (20 mg/ml). Insoluble in water.

InChI:InChI=1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H

InChI Key:MHMNJMPURVTYEJ-UHFFFAOYSA-N

Acidity coefficient:9.31 (Predicted)

Refractive index:1.753

Vapor Pressure:9.53E-21mmHg at 25°C

Storage condition:-20ºC for long time

Application:Fluorescein 5(6)-isothiocyanate (CAS:27072-45-3) is an isothiocyanate-derivitized Fluorescein (sc-215040, sc-206026) fluorescent dye compound used for immunofluorescent identification and immunofluorescent histology applications. Fluorescein 5(6)-isothiocyanate reacts with nucleophilic amine and thiol amino acid residues to covalently link the compound to the protein of investigation. Fluorescein isothiocyanates have been described for use in the immunofluorescent identification of microorganisms and for the immunohistochemical localization of proteins.

Reference: 

1. Riggs, J.L., et al. 1958. Amer. J. Pathol., 34: 1081-1097. PMID: 13583098

2. Sweet, G.H. and Schindler, C.A. 1967. J. Bacteriol. 93: 1943-1948. PMID: 5337774

3. Geffen, L.B., et al. 1969. J. Physiol. 204: 593-605. PMID: 4980932

2. Mann, K.G. and Fish, W.W. 1972. Methods Enzymol. 26: 28-42. PMID: 4680709

5. Sakal, E., et al. 1991. Biochemistry. 30: 8899-8904. PMID: 1909577

Packaging & Delivery

Package:1g/5g/10g/25g/50g/bulk package

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