CAS:146-68-9 | Iodonitrotetrazolium Chloride

Name:Iodonitrotetrazolium chloride

Synonyms:2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium Chloride; 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium Chloride; 2-(p-Iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazolium Chloride; 3-(p-Nitrophenyl)-2-(p-iodophenyl)-5-phenyltetrazolium Chloride; INT; Iodonitro Tetrazolium; Iodonitrotetrazolium Purple; Iodonitrotetrazolium Violet; NSC 27620; p-Iodonitrotetrazolium Violet

CAS:146-68-9

Purity:95%

Molecular Formula:C₁₉H₁₃ClIN₅O₂

Molecular Weight:505.70

Melting Point:231ºC

Boiling Point:567.5ºC at 760 mmHg

Flash Point:297ºC

PSA:80.41

LogP:1.25

Appearance:slightly yellow powder

Stability:Stable. Incompatible with strong oxidizing agents

Sensitivity:Light Sensitive

Solubility:methanol: water (1:1): 50 mg/mL hot, very faintly turbid, very deep yellow

Water solubility:soluble

Vapor Pressure:6.76E-13mmHg at 25°C

Storage condition:Store at 2-8ºC

Application:Iodonitrotetrazolium chloride(CAS:146-68-9) is an electron acceptor used in various colorimetric assays. The chemical has been mainly documented for its measurement of dehydrogenase activity. In the assay to measure SDH (succinate dehydrogenase) activity, Iodonitrotetrazolium chloride was used as an oxidizing agent being reduced by succinate dehydrogenase. Upon reduction the compound forms a formazan dye measured at 500 nm. Iodonitrotetrazolium chloride has also been used to study the electron transport activity in soils, sediments, and pure enzyme cultures as well as nucleotide diphosphate kinase activity.

Reference: 

 1. Trevors, J.T. 1984. Crit. Rev. Microbiol. 11: 83-100. PMID: 6375976

2. Munujos, P., et al. 1993. Anal. Biochem. 212: 506-509. PMID: 8214593

3. Kamburova, M. 1993. Talanta. 40: 707-711. PMID: 18965691

4. Mailloux, R.J., et al. 2008. Electrophoresis. 29: 1484-1489. PMID: 18324728

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